Fig. 4. MON-PEI ameliorates TNBS-induced chronic colitis in vivo.

(A) Experimental scheme for TNBS-induced chronic acute colitis and scavenger (MON, PEI, and MON-PEI) administration. (B to D) Daily body weight, (C) colon length, and (D) colonic damage scores of mice in each group via rectal enema were monitored at day 5. (E) Representative images of colon sections of indicated groups via oral gavage were stained with H&E. Scale bars, 100 μm. (F) Body weight, (G) colon length, and (H) colonic damage scores of MON-PEI–treated mice via oral gavage, intraperitoneal injection, and rectal enema were determined at day 5. Data are means ± SEM (n = 6 to 8 mice per group; *P < 0.05, **P < 0.01, and ***P < 0.001 by one-way ANOVA with Tukey’s multiple comparison test).