Figure 3.

After treatment with different concentrations of Hu, FH protein expression increased in the FH overexpression group (A). However, in the FH expression knockdown group (B), FH expression first increased and then decreased. The ROS level increased slightly in the FH overexpression group (C), but in the FH expression knockdown group (D), the ROS level increased significantly after treatment with Hu. After treatment with different concentrations of Hu, apoptosis increased and proliferation decreased in the FH overexpression group (E,F). However, the differences were not obvious. In the FH expression knockdown group, apoptosis increased and proliferation decreased significantly. FH, fumarate hydratase; ROS, reactive oxygen species.