Figure 4.

MiR-29b suppresses the invasion and migration of HCC cell lines. (A) Control, miR-29b mimic-, miR-NSM- and miR-29b inhibitor-transfected HepG2 and Huh7 cells were subjected to wound healing assay, and the distance of migration was measured at 12 and 24 h. N=3 in each group; scale bar, 200 µm. (B) Cells were grown in a 24-well plate coated with Matrigel Matrix, and the cell invasiveness was assessed using a Transwell assay. N=3 in each group; stained with crystal violet, scale bar, 100 µm. Representative images are shown, and all data are presented as means ± standard deviations. *, P<0.05 compared with each group. HCC, hepatocellular carcinoma.