Figure 5.

MiR-29b overexpression promotes apoptosis in HCC cells. Control, miR-29b mimic-, miR-NSM- and miRNA-29b inhibitor-transfected cells were grown to 80–90% confluence. (A) Hoechst 33342 staining of cells and apoptosis percentages quantified by Hoechst 33342 staining. (B) TUNEL staining of cells and statistical analysis. Arrows indicate the apoptotic cells. All images are at ×100 magnification, and the arrows indicate the apoptotic nuclei. Data are shown at least 3 independent experiments, *, P<0.05 compared with each group. All data are expressed as means ± standard deviations. HCC, hepatocellular carcinoma.