Figure 1.

ONECUT2 mRNA expression in prostate and lung cancer model systems. LNCaP, VCaP, DU145, and 22Rv1 are commonly used cell line models. EF1 is derived from the MSKCC PCa4 neuroendocrine organoid line. H660 is a human neuroendocrine prostate cancer line. Transformed PARCB models are human prostate basal epithelial cells genetically engineered with dominant negative p53 (TP53DN) (P), myrAKT1 (A), RB1-shRNA (R), c-Myc (C) and BCL2 (B). PARCB tumors show histologic and biochemical features of small cell prostate cancer. PACB (without RB1-shRNA), ARCB (without TP53DN), and ACB (without TP53 and RB1-shRNA) displayed histological features of poorly differentiated prostate adenocarcinoma. PACB and ARCB tumors displayed only focal expression of neuroendocrine markers. Primary NHBE cells transformed with the PARCB lentiviruses in a small cell lung cancer model show increased ONECUT2 expression. All data shown are from reference 6. NHBE, normal human bronchial epithelial; shRNA, short hairpin RNA; TPM, transcripts per million.