Skip to main content
. 2021 Dec 31;10:e67282. doi: 10.7554/eLife.67282

Figure 4. SSNA1 binds cooperatively to microtubules.

Representative kymographs of single-molecule SSNA1 binding events on GMPCPP-stabilized microtubules and corresponding quantification of the single-molecule dwell times (A) 0–5 min and (B) 5–10 min after SSNA1 addition. Representative kymographs of single-molecule SSNA1 binding events in the presence of excess SSNA1 (‘spiking’ condition) on GMPCPP-stabilized microtubules and corresponding quantification of the single-molecule dwell times (C) 0–5 min and (D) 5–10 min after SSNA1 addition. (E) Cumulative distribution plots of SSNA1 single-molecule dwell times at 0–5 min and 5–10 min post-addition of SSNA1 for both the single-molecule control (blue dots) and spiking (purple dots) conditions.

Figure 4—source data 1. An Excel sheet containing numerical data for the quantification of single-molecule SSNA1 dwell times and SSNA1 fluorescence intensity, as presented in Figure 4 and Figure 4—figure supplement 1.

Figure 4.

Figure 4—figure supplement 1. SSNA1 coats GMPCPP-stabilized microtubules.

Figure 4—figure supplement 1.

Representative images showing GMPCPP-stabilized microtubules incubated with 2 µM Alexa-488-SSNA1 at 0, 2, and 5 min after SSNA1 addition. The total SSNA1 fluorescence intensity along the total length of microtubule lattice in the microscope field of view excluding the background was measured over time for four independent experimental repeats.
Figure 4—video 1. SSNA1 coats GMPCPP-stabilized microtubules.
Download video file (548.6KB, mp4)
GMPCPP-stabilized microtubules (red) were incubated with 2 µM 488-SSNA1 (cyan). Time is in min:s. Scale, 10 µm, Playback, 10 fps.