Figure 3.

OPN over-expression contributes to acquired resistance to afatinib in H1650-AR and H1975-AR cells. (A) The pLOC-OPN lentivirus vector was used to over-express OPN in H1650-AR and H1975-AR cells. Empty vector was used as a control. The expression level of OPN protein was measured by western blot. (B) H1650-AR and H1975-AR cells with OPN over-expression or empty vector control were administrated with afatinib (2.5, 5.0, 10, 20, 50, 100 µmol/L) for 48 h; Then cell proliferation inhibition assay was carried out. Each experiment was repeated at least 3 times independently. Data was represented as mean ± SD.*P<0.01, vs. control. OPN, osteopontin.