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. 2019 Apr;8(2):410–421. doi: 10.21037/tcr.2019.02.03

Figure 2.

Figure 2

RHAMM expression in A549 cell line following gene knockdown. (A) Detection of RHAMM mRNA expression in A549 cells using RT-qPCR showing lower levels in the siRHAMM group compared with the Control and NC groups; (B) RHAMM protein expression detection by western blot 48 h post-transfection. Expression of RHAMM protein 48 h post-transfection was also reduced. *, P<0.05 vs. control group at 24 h; #, P<0.05 vs. NC group at 24 h; $, P<0.05 vs. control group at 48 h; &, P<0.05 vs. NC group at 48 h. The RHAMM-siRNA 2 sequence, which reduced RHAMM mRNA and protein levels by 84% and 93%, respectively, at 48 h post-transfection, was used for subsequent experiments. RHAMM, receptor for hyaluronan-mediated motility; Control, mock transfection; NC, negative control; siRNA, small interfering RNA; siRHAMM, RHAMM knockdown.