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. 2021 Feb;10(2):790–805. doi: 10.21037/tcr-20-2597

Figure 2.

Figure 2

Silencing Rab25 inhibits gastric adenocarcinoma cells proliferation in vitro. (A) qPCR analysis comparing Rab25 mRNA expression (normalized to β-actin) in gastric adenocarcinoma cells with normal gastric mucosal epithelial cell lines GES-1. ***, P<0.001; ns, not statistically significant. (B) qPCR analysis (normalized to β-actin) and western blot analysis (normalized to β-Actin) of Rab25 expression in AGS cells transfected with control shRNA (Rab25 shn.c) or two different shRNAs against Rab25 (shRab25-1 and shRab25-2). ***, P<0.001. (C) The growth curves of the Rab25 shn.c and shRab25 AGS cells by survival cell count assay in vitro. *, P<0.05; **, P<0.01. (D) Representative images (left) and quantification (right) of colony formation in six-well plates from the Rab25 shn.c and shRab25 AGS cells. ***, P<0.001. Stained by coomassie brilliant blue dye and clones in 6-well plate without magnificatio. (E) Representative images (left) and quantification (right) of colony formation per 10-fold field of view in soft agar from the Rab25 shRNA knockdown AGS cell lines. ***, P<0.001. Stained and magnified ten times.