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. 2021 Feb;10(2):790–805. doi: 10.21037/tcr-20-2597

Figure 6.

Figure 6

Rab25 up-regulation promotes phosphorylation of PKM2. (A) Western blot analysis (normalized to β-Actin) of PKM2-pTyr, PKM2, and Rab25 protein levels in the pHBLV-n.c and pHBLV-Rab25 GES-1 cells. (B) Western blot analysis (normalized to β-Actin) of PKM2-pTyr, PKM2, and Rab25 protein levels in the Rab25 shn.c and shRab25 AGS cells. (C) Western blot analysis (normalized to β-Actin) of PKM2-pY105, PKM2, and Rab25 protein levels in the pHBLV-n.c and pHBLV-Rab25 GES-1 cells. (D) Western blot analysis (normalized to β-Actin) of PKM2- pY105, PKM2, and Rab25 protein levels in the Rab25 shn.c and shRab25 AGS cells. (E) An assumed diagram of the mechanism of Rab25 regulating glycolysis pathway in gastric adenocarcinoma cells.