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. 2020 Mar;9(3):1742–1751. doi: 10.21037/tcr.2020.03.26

Figure 1.

Figure 1

Low expression of PTEN and the promoting effect of LSZ on PTEN in LC. A. The expression of PTEN was detected by IHC in cancer tissue and normal tissue. B. The mRNA level of PTEN was detected by RT-qPCR in human lung fibroblasts (MRC-5) and human LC cells (H1650, A549, H1299, and PC-9). (*, P<0.05; **, P<0.01 vs. MRC-5 cells) C. The protein level of PTEN was detected by western blotting in MRC-5, H1650, A549, H1299, and PC-9 cells. (*, P<0.05; **, P<0.01 vs. MRC-5 cells) D. Cytotoxic effect of LSZ on MRC-5 was detected by CCK8 assay. (*, P<0.05; **, P<0.01; ***, P<0.001 vs. control group) E. The mRNA level of PTEN was detected by western blotting in H1299 cells treated with different doses of LSZ (0, 10, 20, 40, 60, 80, 100, and 120 mg/kg). F. The protein level of PTEN was detected by western blotting in H1299 cells treated with different doses of LSZ (0, 10, 20, 40, and 60 mg/kg). (*, P<0.05; **, P<0.01; ***, P<0.001 vs. control group).