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. 2019 Feb;8(1):35–43. doi: 10.21037/tcr.2018.12.26

Figure 5.

Figure 5

BLACAT1 silencing suppresses proliferation and expression of EMT markers in CRC. HCT116 cells were transfected with si-NC or si-BLACAT1-1 + si-BLACAT1-2 for 48 h, following which proliferation and expression of EMT markers were determined using western blotting and qRT-PCR. (A) Protein levels of cyclin D1 and CDK6 were determined using western blotting and (B) mRNA levels were quantitated using qRT-PCR. (C) Protein levels of E-cadherin and vimentin were analyzed using western blotting and (D) mRNA levels were determined using qRT-PCR. Data show mean ± SEM. *, P<0.05; **, P<0.01 compared to the control group. CRC, colorectal cancer; qRT-PCR, quantitative reverse transcription-polymerase chain reaction.