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. 2021 May;10(5):2437–2450. doi: 10.21037/tcr-21-689

Figure 3.

Figure 3

Curcumol increases the 5-FU-sensitivity of HCT116/5-FU cells by modulating the Wnt/β-catenin pathway. (A) Effects of 5-FU (range from 0 to 60 µM) on HCT116 and HCT116/5-FU cell viability (n=6). (B) HCT116/5-FU cell apoptosis was detected 48 hours after treatment with curcumol (30 µg/mL) and/or 5-FU (20 µM) using flow cytometry analysis (n=6). (C) Flow cytometry results are showed as quantitative bar graphs. Mortality was defined as the percentage of Annexin V+ cells (n=6). (D) Representative western blots were depicting the levels of the Bad, Bcl-2, MRP-2, P-gp, Survivin, β-Catenin, and p-β-Catenin proteins in the indicated colon cancer cell lines. (E) The Bad/Bcl-2 ratio was quantitatively analyzed (n=6). (F) The protein expression levels were quantitatively analyzed (n=6). All experimental data are presented as the means ± SD. Statistical significance: *P<0.05 and **P<0.01 compared with the HCT116/5-FU group; ##P<0.01 compared with the HCT116/5-FU+5-FU group. IC50: the 50% inhibitory concentration. 5-FU, 5-fluorouracil.