TABLE 1.
ECL counts obtained by NASBA assay and CFU counts obtained by quantitative LightCycler-PCR (LC-PCR) for A. fumigatus in defined dilutions for different filamentous fungi and for C. albicans, cytomegalovirus, and human RNA
| Inoculum (CFU)a | Mean ECL count ± SD as determined by NASBA assay | Mean CFU/ml ± SD as determined by LC-PCRb |
|---|---|---|
| Aspergillus fumigatus (105) | 82,841 ± 52,282 | 100,650 ± 38,112 |
| Aspergillus fumigatus (104) | 61,770 ± 46,673 | 11,230 ± 5,416 |
| Aspergillus fumigatus (103) | 32,901 ± 18,073 | 990 ± 523 |
| Aspergillus fumigatus (102) | 7,201 ± 11,412 | 125 ± 68 |
| Aspergillus fumigatus (101) | 3,133 ± 3,067 | 9.3 ± 0.1 |
| Aspergillus fumigatus (1) | 2,891 ± 3,450 | Neg |
| Aspergillus flavus∗ | 230,117 | ND |
| Aspergillus niger∗ | 293,710 | ND |
| Aspergillus versicolor∗ | 138,254 | ND |
| Aspergillus glaucus∗ | 123,072 | ND |
| Scopulariopsis brevicaulis∗ | 325 | Neg |
| Curvularia inaequalis∗ | 134 | Neg |
| Absidia corymbifera∗ | 134 | Neg |
| Fusarium solani∗ | 146 | Neg |
| Rhizopus oryzae∗ | 180 | Neg |
| Acremonium chrysogenum∗ | 130 | Neg |
| Penicillium brevicompactum∗ | 17,519 | Pos |
| Penicillium chrysogenum∗ | 44,226 | Pos |
| Alternaria alternata∗ | 844 | Pos |
| Candida albicans∗ | 196 | Neg |
| Cytomegalovirus | 134 | Neg |
| Human fibroblast | 209 | Neg |
| Double-distilled water | 90 ± 66 | Neg |
a
Organisms tested in an undiluted suspension are indicated with an asterisk.
b
Neg, negative; Pos, positive; ND, not done.