| Afford |
Company |
Laboratory, company |
| Technology limitation |
Short sequence reads, not map uniquely to a single gene or isoform (mapping uncertainty), |
Background hybridization, cross-hybridization, specific hybridization, probe design |
| Testing dynamic range |
Wider (low to extremely abundant, more sensitive, accurate) |
Cannot distinguish ‘‘no’’ from ‘‘low’’ expression. |
| Data analysis |
Far from mature, vulnerable to general biases and errors, the absence of a gold standard for analysis |
Relatively simple, faster |
| Cost |
Expensive |
Cheap |
| Advantage |
Can discover unknown transcripts and rare transcripts, higher detection throughput, highly reproducible, with relatively little technical variation |
Can provide highly consistent data, the analysis software is quite mature, and give clinical diagnostic value |
