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. 2020 Apr;9(4):2482–2491. doi: 10.21037/tcr.2020.03.17

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2020 Translational Cancer Research. All rights reserved.

Open Access Statement: This is an Open Access article distributed in accordance with the Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License (CC BY-NC-ND 4.0), which permits the non-commercial replication and distribution of the article with the strict proviso that no changes or edits are made and the original work is properly cited (including links to both the formal publication through the relevant DOI and the license). See: https://creativecommons.org/licenses/by-nc-nd/4.0/.

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Denosumab inhibits MCF-7-induced spontaneous osteoclastogenesis of RAW 264.7 cells. (A) Co-culture system for spontaneous osteoclastogenesis of RAW 264.7 cells induced by MCF-7 cells; (B) representative field of co-culture after TRAP staining and statistics of TRAP+ osteoclastogenesis; (C,D) the mRNA expression of bone resorption gene (C) and osteoclast phenotype gene (D). Three times for each experiment and ***, P<0.001 vs. control group. Scale bar =20 mm. TRAP, tartrate-resistant acid phosphatase.