TABLE 1.

Results of light microcopy, PCR amplification, digestion of PCR products, and DNA sequencing of amplified PCR products from nine patients with microsporidiosis

Patient no.	Microsporidian species	Detection of spores by light microscopy	Length (bp) of DNA fragment(s)			% Homologyb
			After PCRa	After digestion with:
				PstI	HaeIII
1	E. bieneusi	+	250	250	208	99.2d
2	E. bieneusi	+	250	250	208	99.6d
3	E. bieneusi	+	250	250	208	99.6d
4	E. bieneusi	−	250	250	208	99.6d
5	E. bieneusi	−	250	250	208	ND
6	Encephalitozoon sp.	+	270	NDc	ND	ND
7	Encephalitozoon sp.	+	270	ND	ND	ND
8	E. bieneusi	−	250	250	208	ND
	E. hellem		279	133 + 146	200	99.6e
9	E. bieneusi	−	250	250	208	99.6d
	E. hellem		279	133 + 146	200	99.6e

^aLength of DNA fragments amplified with primer pair VI/PMP2. 

^bHomology with the indicated DNA sequence from GenBank. 

^cND, not done. 

^dThe E. bieneusi SSU rRNA sequence (accession no. AF024657) was used for comparison. 

^eThe E. hellem SSU rRNA sequence (accession no. AF039229) was used for comparison.