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. 2022 Feb;28(2):373–381. doi: 10.3201/eid2802.211874

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Emerging Infectious Diseases is a publication of the U.S. Government. This publication is in the public domain and is therefore without copyright. All text from this work may be reprinted freely. Use of these materials should be properly cited.

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Detection of infectious hypodermal and hematopoietic necrosis virus (IHHNV) in Penaeus vannamei shrimp from the United States by conventional PCR, 2019. Agarose gel photographs show 389-bp IHHNV–specific amplicon (left column) and 309-bp IHHNV–specific amplicon (right column). Top row represents case number 19-490, P. vannamei broodstock samples originating in Florida (A and B). Middle row represents case number 19-428, P. vannamei post-larvae samples originating in Texas (C1 and C2). Bottom row represents case number 19-644, frozen P. vannamei shrimp originating from an indoor farm in Texas (A, B, C, and D). Lane M, 100-bp molecular weight marker (New England Biolabs, Inc., https://www.neb.com); lane SPF, specific pathogen-free P. vannamei shrimp; lane NT, no template control; lane +C, positive control for PCR.