Table 1. Primers used for the detection of the IHHNV genome in commercially raised Penaeus vannamei shrimp, Texas and Florida, United States, 2019*.
| Target gene | Primer name | Sequence, 5′ → 3′ | Amplicon size, bp | Reference |
|---|---|---|---|---|
| NS1 | 389F | CGGAACACAACCCGACTTTA | 389 | (13) |
| NS1 | 389R | GGCCAAGACCAAAATACGAA | ||
| NS1 | IHHNV-309F | TCCAACACTTAGTCAAAACCAA | 309 | (13) |
| NS1 | IHHNV-309R | TGTCTGCTACGATGATTATCCA | ||
| NS1 (Left) | 159_IH_NS1_S | AACTGACGAGTGAAGAGGCT | 1295 | This study |
| NS1 (Left) | 1446_IH_NS1_AS | GTGTCCGGAGTATGTGATGT | This study | |
| NS1 (Right) | 1400_IH_NS1_S | GGACGAACGCCAAACTTCAC | 1313 | This study |
| NS1 (Right) | 2698_IH_NS1_AS | ATCTGTGTGGGTCTGGTCC | This study | |
| CP | CP1F | GATCACCAGCACGACTTCCT | 1088 | (21) |
| CP | CP2R | CGGGTATATATTGCACATCGAA |
*CP, capsid protein gene; IHHNV, infectious hypodermal and hematopoietic necrosis virus; NS1, nonstructural gene 1.