Table 2. Additional primers designed and used for Sanger sequencing of CP and NS1 genes of IHHNV, Texas and Florida, United States, 2019*.

Target gene	Primer name	Sequence, 5′ → 3′
NS1	159_IH_NS1_S	AAC TGA CGA GTG AAG AGG CT
NS1	1446_IH_NS1_AS	GTG TCC GGA GTA TGT GAT GT
NS1	482_IHHNV_NS1_S	CCC CAA CAA ATA TCG CTG CG
NS1	511_IHHNV_NS1_S	AGA TCA CAT TCT ACC GTG GTG
NS1	342_IH_NS1_Seq_AS	ACTT GTA CTT ACA TTT GTA T
NS1	1400_IH_NS1_S	GGA CGA ACG CCA AAC TTC AC
NS1	2627_IH_NS1_Seq_S	CAA GCC CAA GGA AAA GAT CC
NS1	2390 CP_S	CTA CTG GGT ACC ACC AGC
NS1	2698 IH_NS1_AS	ATC TGT GTG GGT CTG GTC C
NS1	2540 IH_CP_S	AGG CCT CTT CCA AGA ATA CG
NS1	2682 IHHNV_NS1_AS	ACT TGA TCC TTC GGC GTG TT
NS1	2540 IH_CP_S	AGG CCT CTT CCA AGA ATA CG
NS1	2682 IHHNV_NS1_AS	ACT TGA TCC TTC GGC GTG TT
NS1	IHHNV-1942_F	GTC ACT AAT TAC AAA CCT GCA G
NS1	IHHNV-2020R	GCA TAT TGT CGT AGT CTG GT
CP	IHHNV-CP_S	ATG TGC GCC GAT TCA ACA AG
CP	IH_CP_Seq_S	CAT AAT CAA CTA TCA ACT AA
CP	IH_CP_Seq_AS	TGC CAA TGT TAC GTC GGT TTC C

*CP, capsid protein gene; IHHNV, infectious hypodermal and hematopoietic necrosis virus; NS1, nonstructural gene 1.