Table 5. Relationship between dose and competence of Anopheles quadrimaculatus mosquitoes for Cache Valley virus, New York, USA, 2000‒2016*.
| Strain | Day postinfection | Blood meal titer log10 PFU/mL | No. infected/no. tested (%) | No. disseminated/no. tested (%) | No. transmitted.no. tested (%) |
|---|---|---|---|---|---|
| L1-NY10 | 15 | 5.1 | 11 /25 (44) | 10/11 (90.91) | 0/10 (0) |
| 4.2 | 3/25 (12)† | 2/3 (66.67) | 0/2 (0) | ||
|
|
|
3 |
0/25 (0) |
NT |
NT |
| L1-NY25 | 15 | 6.8 | 24/25 (96) | 22/24 (91.67) | 1/22 (4.55) |
| 5.7 | 13/25 (52) | 12/13 (92.31) | 1/12 (8.33) | ||
|
|
|
4.5 |
3/25 (12)† |
0/3 (0)‡ |
NT |
| L2–15350152 | 15 | 5.1 | 18/25 (72) | 18/18 (100) | 0/18 (0) |
| 4.3 | 16/25 (64) | 16/16 (100) | 2/16 (12.50) | ||
|
|
|
3 |
7/25 (28) |
7/7 (100) |
1/7 (14.29) |
| L2–15330577 | 15 | 5.7 | 19/25 (76) | 19/19 (100) | 8/19 (42.11) |
| 5 | 15/25 (60) | 15/15 (100) | 7/15 (46.67) | ||
|
|
|
3.7 |
7/25 (28) |
7/7 (100) |
2/7 (28.57) |
| R-15041084 | 15 | 5.3 | 15/25 (60) | 15/15 (100) | 1/15 (6.67) |
| 4.4 | 13/25 (52) | 13/13 (100) | 1/13 (7.69) | ||
|
|
|
3.2 |
5/25 (20) |
3/5 (60) |
0/3 (0) |
| R-PA | 15 | 4.9 | 10/25 (40) | 0/10 (0) | NT |
| 3.7 | 6/25 (24) | 0/6 (0) | NT | ||
| 2.9 | 0/25 (0) | NT | NT |
*L1, lineage 1; L2, lineage 2; NT, not tested; R, reassortant (containing lineage 1 large RNA segment and lineage 2 small and medium RNA segments); NT, not tested. †p<0.05: lineage 1 strain infection rate compared with lineage 2 (15350152) strain when mosquitoes were fed on infectious blood ≈4–4.5 log10 PFU/mL titer. ‡p<0.05: lineage 1 strains dissemination rate compared with lineage 2 strains when mosquitoes were fed on infectious blood ≈4–4.5 log10 PFU/mL titer.