Table 6. Infection, dissemination, and transmission rates for Anopheles quadrimaculatus mosquitoes for different Cache Valley virus isolates, New York, USA, 2000‒2016*.
| Strain | Blood meal titer, log10 PFU/mL | Day postinfection | No. infected/no. tested (%) | No. disseminated/no. tested (%) | No. transmitted/no. tested (%) |
|---|---|---|---|---|---|
| L1-NY10 | 7.1 | 2 | 25/25 (100) | 18/25 (72)† | NT |
| 6 | 42/42 (100) | 42/42 (100) | 5/42 (11.90) | ||
|
|
|
15 |
41/41 (100) |
41/41 (100) |
5/41 (12.20) |
| L1-NY25 | 6.6 | 2 | 18/25 (72)‡ | 3/18 (16.67)† | NT |
| 6 | 31/35 (88.57)‡ | 16/31 (51.61)† | 0/16 (0) | ||
|
|
|
15 |
32/35 (91.43)‡ |
23/32 (71.88)† |
4/23 (17.39) |
| L2–15350152 | 7.2 | 2 | 25/25 (100) | 25/25 (100) | NT |
| 6 | 37/37 (100) | 37/37 (100) | 11/37 (29.73) | ||
|
|
|
15 |
44/44 (100) |
44/44 (100) |
24/44 (54.54) |
| L2–15330577 | 7.1 | 2 | 25/25 (100) | 25/25 (100) | NT |
| 6 | 35/35 (100) | 35/35 (100) | 7/35 (20) | ||
|
|
|
15 |
35/35 (100) |
35/35 (100) |
10/35 (28.57) |
| L2-Hu2011 | 6.3 | 2 | 25/25 (100) | 25/25 (100) | NT |
| 6 | 30/30 (100) | 30/30 (100) | 8/30 (26.67) | ||
| 15 | 30/30 (100) | 30/30 (100) | 9/30 (30) | ||
| 5.5 | 2 | 25/25 (100) | 25/25 (100) | NT | |
| 6 | 30/30 (100) | 30/30 (100) | 1/30 (3.33) | ||
|
|
|
15 |
24/24 (100) |
24/24 (100) |
5/24 (20.83) |
| R-15041084 | 7.1 | 2 | 25/25 (100) | 25/25 (100) | NT |
| 6 | 41/41 (100) | 41/41 (100) | 6/41 (14.63) | ||
|
|
|
15 |
34/34 (100) |
34/34 (100) |
18/34 (52.94) |
| R-PA | 5 | 2 | 10/25 (40) | 4/10 (40) | NT |
| 6 | 21/35 (60) | 3/21 (14.29) | 0/3 (0) | ||
| 15 | 30/35 (85.71) | 0/30 (0) | NT | ||
| R-15041170 | 7 | 2 | 25/25 (100) | 25/25 (100) | NT |
| 6 | 30/30 (100) | 30/30 (100) | 1/30 (3.33) | ||
| 15 | 30/30 (100) | 30/30 (100) | 11/30 (36.67) | ||
| 4.7 | 2 | 24/25 (96) | 11/24 (45.83) | NT | |
| 6 | 30/30 (100) | 29/30 (96.67) | 0/29 (0) | ||
| 15 | 25/27 (92.59) | 23/25 (92) | 1/23 (4.34) | ||
| R-15060131 | 7.4 | 2 | 25/25 (100) | 25/25 (100) | NT |
| 6 | 30/30 (100) | 30/30 (100) | 2/30 (6.67) | ||
| 15 | 21/21 (100) | 21/21 (100) | 4/21 (19.05) | ||
| 4.1 | 2 | 25/25 (100) | 17/25 (68) | NT | |
| 6 | 30/30 (100) | 28/30 (93.33) | 1/28 (3.57) | ||
| 15 | 29/30 (96.67) | 26/29 (89.66) | 2/26 (7.69) |
*L1, lineage 1; L2, lineage 2; NT, not tested; R, reassortant (containing lineage 1 large RNA segment and lineage 2 small and medium RNA segments); NT, not tested. †p<0.05: lineage 1 strains dissemination rate compared with lineage 2 strains when mosquito fed on infectious blood ≈6.3–7.4 log10 PFU/mL titer. ‡p<0.05: lineage 1 strain infection rate compared with lineage 2 strains when mosquito fed on infectious blood ≈6.3–7.4 log10 PFU/mL titer.