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. 2021 Dec;10(12):5307–5318. doi: 10.21037/tcr-21-2295

Figure 3.

Figure 3

Bay-299 induces apoptosis in MV4-11 and NB4 cells and this can be rescued by Z-VAD-FMK and necrostatin-2. (A) CCK-8 viability assay in MV4-11 and NB4 cells treated with the indicated inhibitors and Bay-299 (4 µM) for 72 hours; (B) CCK-8 viability assay in MV4-11 and NB4 cells pre-treated with Z-VAD (20 µM) or Nec-2 (10 µM) followed by incubation with Bay-299 (4 µM) for 72 hours; (C) flow cytometry analysis of cell death in MV4-11 and NB4 cells pre-treated with Z-VAD (20 µM) or Nec-2 (10 µM), followed by Bay-299 (4 µM) treatment for 48 hours; (D) Western blot analysis and quantification of PARP, caspase 9, and caspase 3 protein expression in MV4-11 cells pre-treated with Z-VAD (20 µM) or Nec-2 (10 µM), followed by incubation with Bay-299 (4 µM) for 48 hours. *, P<0.05; **, P<0.01; ***, P<0.001; ****, P<0.0001. DMSO, dimethyl sulfoxide; PARP, poly adenosine diphosphate-ribose polymerase.