Table 3. Sequences for the bDNA signal amplification probes.
| Items | Function [copies] | Sequence [5'-3'] | Complement [copies] |
|---|---|---|---|
| bDNA probes for EpCAM and CK8/18/19 | Capture probe tail [1] | CTACAAACAAACAATATT | Preamplifier leader [1] |
| Preamplifier repeat [5] | CGCAGCCTCAGCC | Amplifier leader [1] | |
| Amplifier repeat [5] | CCCAGACCCTACC | Label probe [1] | |
| bDNA probes for vimentin and twist | Capture probe tail [1] | CTTCTCAATAACTAACAT | Preamplifier leader [1] |
| Preamplifier repeat [5] | GACGGTCGGCGTT | Amplifier leader [1] | |
| Amplifier repeat [5] | GTCACCGCTCCAC | Label probe [1] | |
| bDNA probes for CD45 | Capture probe tail [1] | GTAAAAAGAAAGGTATAA | Preamplifier leader [1] |
| Preamplifier repeat [5] | AATTATACATCTC | Amplifier leader [1] | |
| Amplifier repeat [5] | GAAATGAATGAAT | Label probe [1] | |
| bDNA probes for CXCR4 | Capture probe tail [1] | CTTTATACCTTTCTTTCA | Preamplifier leader [1] |
| Preamplifier repeat [5] | GCGCGCTGTAGGG | Amplifier leader [1] | |
| Amplifier repeat [5] | AGGCGAGGGGAGA | Label probe [1] |
bDNA, branched DNA.