Table 3. Polymerase chain reaction primer sequence of this experiment.

Objects	Primer sequences	Amplified fragment (bp)	Annealing temperature (°C)
ITIH4	Forward 5′-GGTCTCATCCCGATTTGC-3′, reverse 5′-TGATCCCTGGGTAGGTCAT-3′	155	58
IL-6	Forward 5′-GACAGCCACTCACCTCTTCA-3′, reverse 5′-CGCAGAATGAGATGAGTTGT-3′	457	56
GAPDH	Forward 5′-AACGGATTTGGTCGTATTG-3′, reverse 5′-GGAAGATGGTGATGGGATT-3′	208	58

ITIH4, inter-α-trypsin inhibitor heavy chain 4; IL-6, interleukin-6; GAPDH, glyceraldehyde 3-phosphate dehydrogenase.