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miR-596 negatively regulates SOX4 expression. (A) Potential binding sites between miR-596 and SOX4. (B) RT-PCR analysis of SOX4 mRNA levels relative to U6. Relative SOX4 levels in MNK28 and SGC7901 cells were arbitrarily designated as 1. Each column represents the mean ± SD from three biological repeats. (C) MNK28 and SGC-7901 cell viability. Cells were transfected with PEGFP-N1-miR-596 and NC, and analyzed by MTT assay at 12, 24 and 48 h. *, P<0.05; **, P<0.01.
