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. 2021 Sep;10(9):4158–4168. doi: 10.21037/tcr-21-1502

Figure 2.

Figure 2

The effects of lnc CCHE1 and sh-CCHE1 on BCPAP cell proliferation, cell invasion and migration in vitro. (A) Proliferation of BCPAP cells was detected in the blank, non-sense, lnc CCHE1, and sh-CCHE1 transfected cells using the CCK-8 assay. (B,C) Colony formation also was detected in the blank, non-sense, lnc CCHE1, and sh-CCHE1 transfected cells. (D,E) A transwell assay was used to assess the invasion ability in blank, non-sense, lnc CCHE1, and sh-CCHE1 transfected cells. (B) Take photos with a camera. (D) Scale bar: 200 µm. (B,D) Dye with crystal violet. Data in (B,E) are means ± SDs of three independent experiments using PTC cells from different HDs. *, P<0.05. LncRNA-CCHE1, cervical carcinoma high-expressed long non-coding RNA 1; siRNA, small interfering RNA; CCK-8, Cell Counting Kit-8; shRNA, short hairpin RNA; SD, standard deviation; PTC, papillary thyroid carcinoma; HDs, number of cells; OD, optical density; lnc CCHE1, over-expressing lncRNA-CCHE1; sh-CCHE1, knockdown lncRNA-CCHE1.