Figure 2.

Overexpression of PDZRN4 inhibited the proliferation, migration, and invasion of HCT116 cells. (A) HCT116 cells were transduced with lentiviruses (middle) pLVX empty vector and (right) pLVX-PDZRN4. Representative images of (left) untreated cells, pLVX empty vector, or pLVX-PDZRN4 transduced cells were taken under (Phase, top) light microscope and (GFP, bottom) fluorescent microscope (100×). (B) Percentages of cells treated as in Figure 2A tested positive for GFP using FACS. (C) Western blot of PDZRN4 proteins in cells treated as in Figure 2A. (D) mRNA levels of PDZRN4 in cells treated as in Figure 2A. ***P<0.001 for Student’s t-test. (E) Proliferation rates of cells treated as in Figure 2A by cell proliferation assay. *P<0.05, ***P<0.001 for Student’s t-test. (F) Representative images for the clone formation assay of cells treated as in Figure 2A. (G) Numbers of clones formed (Figure 2F). *P<0.05, **P<0.01 for Student’s t-test. (H) Percentage of cells treated as in Figure 2A in different cell cycle phases. (I) Percentages of cells in different cell phases. ***P<0.001 for Student’s t-test. (J) Representative images for transwell migration and invasion of cells treated as in Figure 2A (100×). (K) Number of migrated and invaded cells per field. ***P<0.001 for Student’s t-test. (L) Representative images of wound healing assay for cells treated as in Figure 2A (×100) (M) Percentages of wound closure. ***P<0.001 for Student’s t-test.