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. 2020 Apr;9(4):2721–2733. doi: 10.21037/tcr.2020.02.52

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2020 Translational Cancer Research. All rights reserved.

Open Access Statement: This is an Open Access article distributed in accordance with the Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License (CC BY-NC-ND 4.0), which permits the non-commercial replication and distribution of the article with the strict proviso that no changes or edits are made and the original work is properly cited (including links to both the formal publication through the relevant DOI and the license). See: https://creativecommons.org/licenses/by-nc-nd/4.0/.

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Phorbol-12-myristate-13-acetate (PMA) induced the polarization of THP-1 and U937 cells to M2-polarized tumor-associated macrophages (TAMs) with increased expression level of Arg-1 and IL-1β. (A) Expressions of CD68 (M2-TAMs) and CD204 (M2-TAMs) were detected after PMA treatment by flow cytometry analysis and the percentage of CD68 and CD204 positive cells were increased in the induced M2-TAMs cells. (B) The mRNA level of Arg-1 and IL-1β were increased in induced M2-TAMs cells by RT-qPCR. Data are presented as the mean ± standard deviation (SD). All experiments were conducted in triplicate. *P<0.05; **, P<0.01; ***, P<0.001.