Figure 4.
Effect of prevention and treatment of limonin on mouse breast tumor metastasis by tail vein injection. (A) Bioluminescence analysis was used to determine tumor metastasis over a 45-day period. (B) Quantitative analysis of metastatic cells for use in lung bioluminescence analysis, ***, P<0.001. P values were calculated using a two sided Student’s t test. p/sec/cm2/sr = photons/second/cm2/steradian. (C) Quantitative analysis of incidence of lung metastasis. (D) Histological analyses of breast cancer cell lung metastases with administration of limonin or vehicle control (magnification, 400×).