Table 2. Basic data of the literature on the diagnosis of EOC by ctDNA detection.

First author	Country	Case group (EOC)			Control group		Sample type	Test method	MicroRNA/ctDNA	Cut off value	TP	FP	FN	TN
		Number of cases	Age (years)		Number of cases	Age (years)
Capizzi (19)	Italy	22	64±8 [46–75]		50	42 [35–62]	Plasma	qPCR	ctDNA content	14.5 ng/mL	17	2	5	48
Kamat (20)	U.S.A	164	62 [28–84]		124	53 [33–80]; 46 [29–81]	Plasma	qPCR	ctDNA content	4,500 GE/mL	143	16	21	108
Vanderstichele (21)	Belgium	57	63 [59–73]; 44 [40–47]		55	48 [25–56]; 58 [36–75]	Plasma	LWC	ctDNA content	Unknown	44	5	13	50
Zachariah (22)	Switzerland	21	61 [32–90]		83	53 [21–78]; 58 [22–93]; 34 [25–43]	Plasma	qPCR	ctDNA content	2,653 GE/mL	16	26	5	57
Zhang (23)	China	24	51.96±14.12		24	42.67±12.28; 37.00±13.07	Plasma	qPCR	ctDNA content	8.57 ng/μL	16	5	8	19
Fu (24)	China	93	55.7 [34–82]		125	Unknown	Plasma	SYBR; Green I	ctDNA content	44 μg/L	61	12	32	113
Shao (7)	China	67	57.4 [39–85]		51	55.7 [42–63]; 51.6 [36–68]	Serum	bDNA	ctDNA content	344.4 μg/L	56	8	11	43
Hu (25)	China	61	54.11±1.03		39	51.00±1.46	Plasma	FDM	ctDNA content	0.72 ng/μL	34	2	27	37

qPCR, real-time fluorescence quantitative PCR; LWC, low coverage whole genome sequencing; SYBR Green I, spot fluorescence staining method; bDNA, branch DNA technology; FDM, fluorescence dye method.