Figure 2.

Synergistic effects of gemcitabine combined with Huaier on pancreatic cancer cell apoptosis. Flow cytometric analysis of PaTu8988 cells untreated or treated with gemcitabine (8 µM), Huaier (6 mg/mL) or a combination of these two drugs. (A) Cells were stained with Annexin-V-FITC and analyzed by flow cytometry, with representative flow cytometric images being shown. (B) The percentage of apoptotic cells including early apoptotic cells (annexin V-FITC single-positive cells) and late apoptotic cells (annexin V-FITC/PI double-positive cells) in the total cell population. Compared with the untreated group or single drug groups, *P<0.05; NS, no significance (n=6 per group).