Figure 4.

Huaier increased the ability of gemcitabine to inhibit tumor growth and metastasis in subcutaneous xenograft and lung metastasis nude mice models. A and C: The nude mice bearing PaTu8988 subcutaneous xenografted tumors were treated with normal saline, gemcitabine (1.5 mg/0.1 mL, twice a week, intraperitoneal injection), Huaier (50 mg/0.1 mL, every day, oral gavage) or gemcitabine (1.5 mg/0.1 mL, twice a week, intraperitoneal injection) plus Huaier (50 mg/0.1 mL, every day, oral gavage). (A) The images of tumors removed 4 weeks after treatment with drugs. Meanwhile, the tumor volume (B) was measured at indicated times, and the tumor weight (C) was measured 4 weeks after treatment. (D,E) Lung metastatic nodules in PaTu8988 pancreatic cancer tail vein injection lung metastasis models. (D) Representative images of the lungs and nodules removed 6 weeks after treatment with drugs mentioned above (the red arrows, metastatic lung nodules). (E) The number of tumor metastatic nodules in lung tissues. *, P<0.05 (n=3 per group) compared with the untreated group or single drug treatment group.