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. 2020 Sep;9(9):5190–5199. doi: 10.21037/tcr-20-1083

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2020 Translational Cancer Research. All rights reserved.

Open Access Statement: This is an Open Access article distributed in accordance with the Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License (CC BY-NC-ND 4.0), which permits the non-commercial replication and distribution of the article with the strict proviso that no changes or edits are made and the original work is properly cited (including links to both the formal publication through the relevant DOI and the license). See: https://creativecommons.org/licenses/by-nc-nd/4.0/.

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Hsa-miR-195-5p induces cell cycle arrest at G0/G1 phase by inhibition of cyclin D1 expression and upregulation of p21 expression in TPC-1 cells. (A) Flow cytometry analysis of cell cycle and quantitative representation. (B) Effects of hsa-miR-195-5p mimics on the endogenous cyclin D1 and p21 protein levels. TPC-1 cells were co-transfected with hsa-miR-195-5p mimics or negative control oligonucleotides. The expression of cyclin D1 and p21 was in cells was evaluated by Western blotting. GAPDH was used as loading control. The data were subjected to two-way ANOVA analysis. **, P<0.01 compared with mimic control, ^#, P<0.05, ^##, P<0.01 compared with inhibitor control; NC, negative control oligonucleotides.