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. 2021 Oct;10(10):4478–4488. doi: 10.21037/tcr-21-1940

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2021 Translational Cancer Research. All rights reserved.

Open Access Statement: This is an Open Access article distributed in accordance with the Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License (CC BY-NC-ND 4.0), which permits the non-commercial replication and distribution of the article with the strict proviso that no changes or edits are made and the original work is properly cited (including links to both the formal publication through the relevant DOI and the license). See: https://creativecommons.org/licenses/by-nc-nd/4.0/.

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MCM3AP-AS1 can directly bind with the RNA-binding protein ZFP36. (A) Analysis of MCM3AP-AS1 subcellular location using the lnc ATLAS website. (B) MCM3AP-AS1 levels in the nucleus and cytoplasm. (C) An RNA pull-down assay showing that ZFP36 can bind directly with MCM3AP-AS1. (D) An RIP assay showing that MCM3AP-AS1 is enriched in the lysate pulled down by an antibody recognizing ZFP36. (E) mRNA levels of CCND1, MYC, and MMP1 are significantly decreased in MCM3AP-AS1-silenced cells as assessed using qPCR assay using MDA-MD-231 cells. (F) mRNA levels of CCND1, MYC, and MMP1 are significantly decreased in MCM3AP-AS1-silenced cells as assessed using qPCR assay using ZR-75-30 cells. **, P<0.01; ***, P<0.001. MCM3AP-AS1, maintenance complex component 3 associated protein antisense RNA 1; ZFP36, ZFP36 ring finger protein; lnc, long noncoding; mRNA, messenger RNA; CCND1, cyclin D1; MYC, c-myc; MMP1, matrix metalloproteinase 1; qPCR, quantitative real-time polymerase chain reaction; RIP, RNA immunoprecipitation.