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. 2020 Nov;9(11):6988–7000. doi: 10.21037/tcr-19-2133

Figure 2.

Figure 2

MiR-185-3p could inhibit EMT in GC cells. (A) qRT-PCR analysis of miR-185-3p in AGS and SGC7901 cells after transfection with miR-185-3p mimics, NC mimics, miR-185-3p inhibitor, or NC inhibitor. Transfection with miR-185-3p mimics successfully boosted the expression of miR-185-3p, while transfection with miR-185-3p inhibitor reduced the expression of miR-185-3p in the investigated cells. (B) qRT-PCR analysis of EMT markers in AGS and SGC7901 cells after transfection. In both cell lines, transfection with miR-185-3p mimics could increase the expression level of epithelial marker E-cadherin while reducing the expression level of mesenchymal markers vimentin and N-cadherin. (C) Western blotting analysis of EMT markers in AGS and SGC7901 cells after transfection. The results showed the same trend as the results of qRT-PCR analysis. The quantification of EMT markers was normalized against GAPDH. (D) Cell scratch assay of AGS cells after transfection. Left, images of the migration of cells after incubation for 24 h; right, quantification of the cell migration. Cells transfected with miR-185-3p mimics showed the least migration. (E) Transwell assay of AGS cells after transfection. Left, images of migration cells in the lower chamber; right, cell counts of the migration cells in the lower chamber. Cells transfected with miR-185-3p mimics was least migration. (F) Cell cycle distribution of AGS cells after transfection. Overexpressed miR-185-3p blocked more cells in G0/G1 phase. (G) Cell apoptosis study of AGS cell after transfection. Overexpression of miR-185-5-3p contributed an augment in the cell apoptosis. *, P<0.05; **, P<0.01; ***, P<0.001.