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. 2020 Nov;9(11):6988–7000. doi: 10.21037/tcr-19-2133

Figure 3.

Figure 3

MiR-185-3p could targeting inhibit cathepsin D in GC cells. (A) Prediction of the targeting relationship between miR-185-3p and cathepsin D. (B) Dual-luciferase reporter analysis in AGS cells after transfection. The signal intensity of luciferase was lowest in WT cells transfected with miR-185-3p mimics. (C) qRT-PCR analysis of cathepsin D in AGS cells after transfection. Cells transfected with miR-185-3p mimics showed the lowest level of cathepsin D. (D) Western blotting analysis of cathepsin D in AGS after transfection. Cells transfected with miR-185-3p mimics showed the lowest level of cathepsin D. The quantification of cathepsin D was normalized against GAPDH. *, P<0.05; **, P<0.01.