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. 2021 Aug;10(8):3772–3787. doi: 10.21037/tcr-21-1240

Figure 2.

Figure 2

DLX6-AS1 knockdown inhibited proliferation, migration, invasion, and EMT but induced apoptosis in NSCLC cells. (A-I) A549 and H1299 cells were transfected with si-NC or si-DLX6-AS1. (A) The expression of DLX6-AS1 was detected by RT-qPCR assay after transfection for 48 hours. (B-D) Colony formation assay (B, crystal violet staining) and CCK-8 assay (C,D) were performed to detect the proliferation ability in A549 and H1299 cells after transfection. (E, crystal violet staining) Cell migration was detected by wound healing assay in A549 and H1299 with si-NC or si-DLX6-AS1 transfection. (F,G) A Transwell assay was conducted to measure the cell migration and invasion potential (crystal violet staining, ×200 magnification). (H) Cell apoptosis was detected by flow cytometry in A549 and H1299 after transfection. (I) The protein levels of E-cadherin and N-cadherin were measured using western blot assay at 48 hours post-transfection. *, P<0.05. DLX6-AS1, distal-less homeobox 6 antisense RNA 1; EMT, epithelial-mesenchymal transition; NSCLC, non-small cell lung cancer; RT-qPCR, reverse transcription-quantitative PCR; CCK-8, Cell Counting Kit-8.