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. 2021 Aug;10(8):3772–3787. doi: 10.21037/tcr-21-1240

Figure 7.

Figure 7

DLX6-AS1 knockdown suppressed the growth of NSCLC xenografts through upregulating miR-16-5p and downregulating BMI1. (A-F) H1299 cells infected with sh-NC or sh-DLX6-AS1 lentiviruses were subcutaneously injected into the flanks of mice in sh-NC or sh-DLX6-AS1 group, respectively. (A) Tumor volume was measured every 7 days for total 35 days. (B) Xenograft tumors were resected and weighed on day 35 post-injection. (C,D) The RT-qPCR assay was performed to detect the expression of miR-16-5p (C) and BMI1 mRNA (D) in NSCLC xenograft tumor samples. (E) The protein level of BMI1 in NSCLC xenograft tumors was measured by western blot assay. (F) The expression of BMI1 in xenograft tumors of NSCLC was detected by the immunohistochemical staining method (×200 magnification). (G) Protein levels of E-cadherin and N-cadherin were determined by western blot assay in xenograft tumors of NSCLC. *, P<0.05. DLX6-AS1, distal-less homeobox 6 antisense RNA 1; NSCLC, non-small cell lung cancer; miR-16-5p, microRNA-16-5p; RT-qPCR, reverse transcription-quantitative PCR.