Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Nov 15;28(1):201–211. doi: 10.1038/s41591-021-01576-3

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Extended Data Fig. 6 — a. UMAP projection of subclustered neutrophils from 21 patient samples, coloured by individual patient ID. b-e. Cell speed (length of velocity vectors; b), acceleration (subspaces where velocity undergoes dramatic changes in either in magnitude or direction; c), divergence (outward flux indicating the extent to which a point behaves like a source; d) and curvature (hotspots of abrupt vector field change; e). f. Differentially activated consensus TFs upregulated (positive logFC) or suppressed (negative logFC) post-dexamethasone in at least 3 of 6 patients at t1, and in at least 2 of 3 patients at t2. g-j. Neutrophil states (g) can be distinguished by expression of proliferative marker TOP2A and activation of immaturity-associated TFs ATF4 and JDP2 (h), CD24 splicing kinetics, velocity, expression and immunocytochemistry (representative images are shown; n = 3 for each group; Scale bar, 5 μm) (i), IL-7R (j), Interferon-stimulated genes such as IFITM1 (k), genes involved in prostaglandin synthesis such as PTGS2 (l), and IL-1R2 (m).