Fig. 2. USP6 regulated IM-induced apoptosis.

A K562-R was infected with USP6 gene-interference lentivirus (shUSP6-1, -2, and -3) or control lentivirus (shNC), Western blot analysis was used to detect USP6 expression. B K562-R was infected with shUSP6-1 or shNC for 24 h, treated with 1 μM IM or vehicle (DMSO) for 48 h, and stained with Annexin V/PI, and flow cytometry was used to measure apoptosis. C K562 cells were infected with USP6 gene overexpression lentivirus (oeUSP6) or control lentivirus (Vector), and the expression of USP6 was detected by Western blot analysis. D At 24 h after K562 cells were infected with oeUSP6 or Vector, they were treated with IM or DMSO for 48 h, stained, and used to detect apoptosis. *P < 0.05, **P < 0.01, ***P < 0.001.