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. 2022 Jan 28;13:571. doi: 10.1038/s41467-022-28142-w

Fig. 2. Molecular signature and differentiation of SCs.

Fig. 2

a Preparation of 3-week-old Pdgfra-GFP metaphysis-epiphysis region from femur and tibia for scRNA-seq analysis. b, c scRNA-seq of 3-week-old Pdgfra-H2BGFP reporter bone. UMAP plot of colour-coded cell clusters within Pdgfra-GFP+ positive cells (b) and visualization of cell type-specific marker genes (c). d–f Monocle trajectory analysis of BMSC lineage differentiation with coloured cell clusters (d) and representation of individual cluster in trajectory (e). Cell type-specific relative gene expression shown in pseudo-time (f). g, h Representative confocal images of 3-week-old Pdgfrb-CreERT2 R26-mT/mG reporter femur with GFP+ (green) and FABP5+ (red) SCs (arrowheads) near vessel buds (B). Bottom panels show a single optical plane (left) and higher magnifications of the boxed area (centre and right), respectively (g). Vav1-Cre-controlled R26-mT/mG reporter activation (GFP expression) labelling hematopoietic cells and osteoclasts (arrow) but not FABP5+ (red) SCs (arrowheads) (h). Independent animals, g, h (n = 4).