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. 2022 Jan 28;13:571. doi: 10.1038/s41467-022-28142-w

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a Preparation of non-haematopoietic stromal cells from PFD14 and control bone for scRNA-seq analysis. b UMAP plots showing colour-coded cell clusters from control and PFD14 bone. Cell types and numbers per cluster are displayed on the right. c–e Heatmap showing the top 5 marker genes for each cluster (c). Feature blots showing markers for non-haematopoietic bone cells: Acan—chondrocytes (CHO); Igfbp6—fibroblasts (FB); Pdgfrb—bone mesenchymal stromal cells (BMSCs); Col22a1—osteoblast lineage cells (OBs); Emcn—ECs; Rgs5—Smooth muscle cells (SMCs) (d). Comparison of marker gene expression in PFD14 and control samples (e). Haematopoietic cells (HCs-1,2,3,4). f, g UMAP plots showing colour-coded BMSC subclusters from control and PFD14 bone (f). Fabp5 and Mmp9 expressing cells are increased in PFD14 samples (g). h, i UMAP plot showing mpMSCs and SCs in the control and PFD14 mpMSC subcluster (h). Fracture-derived SCs show higher expression of Mmp9 and Fabp5 (i).