Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Jan 28;13:571. doi: 10.1038/s41467-022-28142-w

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 7 — a, b. Monocle trajectory analysis of BMSC lineage cell differentiation path during fracture repair (a). Expression of marker genes is displayed in trajectory. Red arrowhead indicates Mmp9+ and Fabp5+ cells in proximity of mpMSC cluster (b). c–e Tile scan confocal images of PFD14 and age-matched control femur showing ECs (EMCN, blue), BMSCs (PDGFRβ, red) and chondrocytes (ACAN, green) (c). High magnification images showing avascular regions of callus containing ACAN+ chondrocytes and PDGFRβ+ BMSCs associated with vessels (d). Low and high magnification images showing CD31^hi (green) and EMCN^hi (red) vessels and endothelial buds (arrowheads) in proximity of callus chondrocytes. OSX+ (purple) cells (arrows) are abundant around vessels (e). f–j Tile scan confocal image of PFD14 femur showing MMP9 (green) at the vessel front in the callus (arrowheads) and, at lower level, in the metaphysis near growth plate. Dashed lines mark cortical bone (CB), bone marrow (BM), callus area, and growth plate (f). Representative confocal image of PFD14 callus with FABP5+ (green) SCs associated with distal EMCN+ (red) vessels (arrowheads) (g). High magnifications images show MMP9 and MMP13 immunosignals in the region containing SCs (arrowheads) (h, i). Weaker MMP9 signals relative to SCs (white arrowheads) mark CD68+ (blue) OCs (orange arrowheads) in the PFD14 callus (j). c–i (control = 5 and fracture = 4) independent biological samples.