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. 2022 Jan 28;12:1559. doi: 10.1038/s41598-022-05339-z

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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HNC cells demonstrate the greatest uptake of GNPs in lysosomes. Representative images of cells labelled with 25 µM GNPs. Lysosomes are indicated with hashed outlines. (a) FaDu; (b) HSC-3; (c) Detroit-562; (d) SK-N-AS; (e) Panc1; and (f) A673 cells. Cells were fixed approximately 36 h after GNP-labelling (approximate radiation timepoint) and images were captured by a transmission electron microscope. Imaging was done in triplicate—5 images were captured for each sample, with 3 samples/replicate, and 3 replicates in total. HNC head and neck cancer, GNPs gold nanoparticles.