Table 1.
Box-Behnken design with natural and coded ultrasonication conditions.
| Std | Sonication distance (X1) | Sonication amplitude (X2) | Sonication time (X3) | Sonication power |
|---|---|---|---|---|
| 1 | 0.9 (–1) | 5 (–1) | 2 (0) | 37.5 |
| 2 | 1.5 (1) | 5 (–1) | 2 (0) | 37.5 |
| 3 | 0.9 (–1) | 25 (1) | 2 (0) | 187.5 |
| 4 | 1.5 (1) | 25 (1) | 2 (0) | 187.5 |
| 5 | 0.9 (–1) | 15 (0) | 1 (–1) | 112.5 |
| 6 | 1.5 (1) | 15 (0) | 1 (–1) | 112.5 |
| 7 | 0.9 (–1) | 15 (0) | 3 (1) | 112.5 |
| 8 | 1.5 (1) | 15 (0) | 3 (1) | 112.5 |
| 9 | 1.2 (0) | 5 (–1) | 1 (–1) | 37.5 |
| 10 | 1.2 (0) | 25 (1) | 1 (–1) | 187.5 |
| 11 | 1.2 (0) | 5 (–1) | 3 (1) | 37.5 |
| 12 | 1.2 (0) | 25 (1) | 3 (1) | 187.5 |
| 13 | 1.2 (0) | 15 (0) | 2 (0) | 112.5 |
| 14 | 1.2 (0) | 15 (0) | 2 (0) | 112.5 |
| 15 | 1.2 (0) | 15 (0) | 2 (0) | 112.5 |
| 16 | 1.2 (0) | 15 (0) | 2 (0) | 112.5 |
| 17 | 1.2 (0) | 15 (0) | 2 (0) | 112.5 |