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. 2021 Jun 16;12(1):467–482. doi: 10.1016/j.apsb.2021.05.012

Figure 1.

Figure 1

The distribution of platelets and the expression of P-selectin. (A) Fluorescent images of frozen sections of tumors, lungs and livers collected from 4T1 tumor-bearing mice. The area surrounded by white dotted lines represented metastasis in lungs and livers. Blue: DAPI for staining cell nucleus; red: CD41 for staining platelets. Scale bar = 100 μm. (B) Count of the platelets in tumor free mice and 4T1 tumor-bearing mice. Data are presented as mean ± SD (n = 3), ∗∗P < 0.01. (C) Flow Cytometer detection of P-selectin expression on platelets with or without thrombin activation. Data are presented as mean ± SD (n = 3), ∗∗P < 0.01. (D) Flow Cytometer detection of P-selectin expression on HUVECs with or without the stimulation of 4T1 culture medium. Data are presented as mean ± SD (n = 3), ∗∗∗P < 0.001. (E) Fluorescent images of frozen sections of tumors, lungs and livers collected from 4T1 tumor-bearing mice and tumor free mice. Pink: CD31 for staining neovasculature; red: CD62P for staining P-selectin; blue: DAPI for staining cell nucleus. Scale bar = 100 μm.