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. 2021 Jun 16;12(1):467–482. doi: 10.1016/j.apsb.2021.05.012

Figure 3.

Figure 3

Cellular uptake efficiency and cytotoxicity of preparations on 4T1 cells. (A) Fluorescent images of 4T1 cells after incubating with free DOX, DD/DOX or FD/DOX, respectively, for 1 or 4 h. Red: DOX; blue: DAPI for staining cell nucleus. Scale bar = 100 μm. (B) Flow Cytometer detection of cellular uptake after incubating with free DOX, DD/DOX or FD/DOX, respectively, for 1 or 4 h. Data are presented as mean ± SD (n = 3), ∗∗∗P < 0.001. (C) Fluorescent images of 4T1 cells after incubating with DD/DOX or FD/DOX, respectively, with or without the activated platelet pre-incubation. Red: DOX; blue: DAPI for staining cell nucleus. Scale bar = 100 μm. (D) Flow Cytometer detection of cellular uptake after incubating with DD/DOX or FD/DOX, respectively, with or without the activated platelet pre-incubation. Data are presented as mean ± SD (n = 3), ∗P < 0.05, ∗∗∗P < 0.001. (E) Fluorescent images of 4T1 cells after incubating with DD/DOX or FD/DOX, respectively, for 1 h. Red: DOX; green: Lyso-Tracker Red for staining lysosome; blue: DAPI for staining cell nucleus. Scale bar = 10 μm. (F) Cell viability after incubating with DOX, DD/DOX or FD/DOX, respectively, for 24 h. Data are presented as mean ± SD (n = 3). (G) Cell viability after incubating with DD or FD, respectively, for 24 h. Data are presented as mean ± SD (n = 3).