Anti-tumor and anti-metastasis effect on 4T1 spontaneous metastasis model. (A) Image of 4T1 tumors treated with Glu, Fu, DOX, DD/DOX or FD/DOX (DOX-equivalent 2.5 mg/kg), respectively (n = 5). (B) The changes of tumor volume during treatment. Data are presented as mean ± SD (n = 5), ∗∗∗P < 0.001. (C) The weight of tumors after treatment. Data are presented as mean ± SD (n = 5), ∗∗∗P < 0.001. (D) H&E staining and ki67 staining for tumors. Scale bar = 100 μm. (E) Images of lungs in different groups. The yellow circles represented metastatic nodules on lungs. (F) Count of metastatic nodules on lungs. Data are presented as mean ± SD (n = 5), ∗∗P < 0.01, ∗∗∗P < 0.001. (G) H&E staining for lungs and livers, and the dark purple parts represented tumor metastasis. Scale bar = 100 μm.