Fig. 4.

d-CATH-2 and its derivatives increase BMDM efficiency and slightly enhance the activation by S. suis serotype 2 strains. Mouse BMDM cells were cultured for 6 days. At day 1, 1.25 µM d-CATH-2 or its derivatives were added for 24 h. At day 6 the cells were activated with different S. suis type 2 strains at an MOI of 0.2. Bacteria were mixed for 5 min with 1.25 µM d-CATH-2 or its derivatives before stimulation. After 24 h of stimulation, cells were analyzed by flowcytometry showing median fluorescence index (MFI) (A) and cytokine expression was measured (B). Data is plotted as average +/- SEM (N = 3–6). Samples were compared to no-peptide-controls using two-way ANOVA with the Dunnett post-hoc test. Samples were paired for cell culture samples. *=p ≤ 0.05; **=p ≤ 0.01; ***=p ≤ 0.001; ****=p ≤ 0.0001.